The current study set out to assess how three distinct disinfectants affected the elastomeric imprint material's dimensional stability and antibacterial activity. A consistent stainless steel master die was built in compliance with American Dental Association (ADA) specification number 19. Following the manufacturer's instructions, a total of thirty samples were made on this die using vinyl polysiloxanes (VPS) impression material. Thirty samples were randomly assigned to each of the three groups in order to complete the disinfection process: Group I was diluted water (control group), Group II was 5.25 percent sodium hypochlorite (NaOCl), and Group III was ozone gas. Utilising image investigation software and a stereomicroscope with a 20× magnification, the dimensional stability was assessed. Every disinfectant's antimicrobial effectiveness was evaluated. The control group showed the most dimensions changes in the VPS impression material, followed by 5.25% NaOCl and ozone gas, which showed the lowest dimensional changes. It was discovered that there were statistically considerable differences between the groups. The study's findings suggest that when immersed in various disinfectants, the VPS elastomeric imprint exhibited little dimensional changes. Samples disinfected with 5.25% hypochlorite can be stored for an extended period of time in a clinical setting because the resulting dimensional changes are negligible. Both sodium hypochlorite and ozone gas demonstrated a decrease in the number of germs.