With its capacity to remain dormant inside the host, Mycobacterium tuberculosis (MTB) causes the exceptional illness known as tuberculosis (TB), which has been associated with significant mortality and morbidity for decades. Pulmonary tuberculosis (PTB) is caused by the pathogen infecting the lungs' alveoli, which initiates the condition.Currently, TB ranks within the top ten global deaths. In 2019, 7 million people will be infected with TB worldwide (5,946,816) cases. Karnataka (64,346), one of the top 13 states with the highest TB burden in India, continues to have a significant problem with unfavorable conditions like PTB, which are caused by the original infection in the lungs. Furthermore, urban areas of the state, like Mysuru district (2,544), are among those with a high PTB burden. While TB primarily affects the lungs, it can manifest oral symptoms like ulcerations and swollen lymph nodes, albeit less commonly. Moreover, medications used in TB treatment can have side effects affecting oral health, such as dry mouth and oral candidiasis. Immune suppression resulting from TB can increase susceptibility to oral infections, while poor oral health can potentially exacerbate TB symptoms. Additionally, there's a theoretical risk of TB transmission through saliva if oral lesions are present, though respiratory transmission is more common Objectives: The PTB epidemiology in Mysuru district was the region focused in the study. Methods: For the detection spatial distribution of the infection using GIS and molecular analysis for detection of virulence genes. The regions with the highest incidence and hotspots were targeted. Results: The hotspots zones show the unpredictability of TB transmission in the Mysuru region. A global incidence-based spatial analysis from 2011 to 2019 identified possible TB transmission hotspots and their changes based on the epidemiology study. The Mysuru metropolitan region was found to have the most PTB cases and comes in first among districts with a lot of cases. The staining quantification scale was used to choose the samples, which were then decontaminated and digested. The samples were subjected to direct MTB DNA isolation & PCR amplification. With the help of specific primers and a modified PCR procedure, the dangerous genes fadD33, MmpL10, and WhiB3 were amplified. Conclusion: The DNA isolated from the sample's amplified virulent genes help identify the spread of virulent strains. In some regions with a high incidence of PTB, these findings could be utilized to assess the incidence, dissemination, and circulating virulent strains in society.