A field experiment was conducted during 2018 to 2023. The combined results revealed that treatment T3 followed by T2 exhibited higher values for various parameters such as number of branches per plant, number of leaves per branch, number of leaves per plant, shoot length (cm), leaf yield and stem yield achieving with values of 8.07, 21.17, 178.78, and 128.55 cm for shoot length, as well as 54.84 and 29.59 mt/ha/yr for leaf and stem yield, respectively. Among the ten nutrient levels T2 exhibited significantly higher percentages for nutrient composition such as N, P, K, Ca, Mg and S (3.48, 0.25, 2.21, 0.51, 0.15, and 0.22%) respectively. The same trend was observed for Cu and B (5.11, 101, and 53 ppm). Additionally both T2 and T3 recorded identical values for Zn, Fe, and Mn (47, 101, and 124 ppm). Regarding crude protein, starch, crude fiberand CHO percentages, T2 demonstrated significantly higher values (22, 11.88, 8, and 14.56%) respectively. In terms of total chlorophyll content T2 and T3 exhibited significantly higher values (3 mg/g). Regarding nutrient uptake, T2 recorded significantly higher values for N, Ca, Mg, and S (484, 73, 22, and 32 kg/ha). The uptake values for P and K were the same (35 and 306 kg/ha) in both T2 and T3. While micronutrient uptake T3 recorded higher values for Cu, Zn, Fe, Mn, and B (73, 666, 1424, 1774, and 743 g/ha). The study showed that T3 and T2 exhibited superior performance in all the parameters of mulberry.