Introduction: Platelet-rich plasma (PRP) is defined as a volume of autologous plasma with a platelet concentration higher than the average in peripheral blood. Many trials report that PRP can be used to improve musculoskeletal conditions including osteoarthritis. The correlation between platelet count and clinical response remains uncertain. This study primarily examines platelet count and its indices such as mean platelet volume (MPV), plateletcrit (PCT) and platelet distribution width (PDW) to ascertain their significance in PRP treatment. Methods: PRP was prepared using the BIOPRO PRP kit by Alchem Diagnostics and was administered as an intraarticular injection. Total platelet count, MPV, PCT and PDW of the patient and PRP prepared were measured. The clinical outcomes of the patients were assessed using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and Visual Analog Scale (VAS) grading, before and 6 weeks after the PRP injection Results: The mean age of patients was 48.15±7.02 years and there were 10 (50%) females and 10 (50%) males. An increase in platelet count, MPV and PCT in PRP prepared by double spin method showed improvement in VAS (Before-7.4 ± 0.75; After-4.15 ± 1.18) and WOMAC (Before-74.6 ± 10.98; After-52.85 ± 8.6) were found to be statistically significant. Most of the platelet parameters have a positive correlation with the clinical outcome however it is not statistically significant Conclusion: Intra-articular injection of PRP in OA knee is an efficient and cost-effective treatment. A platelet count of more than 10,000 cells/μL in PRP is sufficient for pain relief