Hypertension, a prevalent cardiovascular disorder, continues to be a significant public health concern globally. This study aimed to evaluate the potential anti-hypertensive activity of the methanolic extract derived from Operculina turpethum Linn Silva Manso stem bark through an in-vivo approach. Male Wistar rats were divided into different groups and subjected to various doses of the methanolic extract. Blood pressure was measured using non-invasive tail-cuff method at regular intervals following extract administration. Additionally, biochemical parameters including lipid profile and renal function tests were assessed to evaluate the safety and efficacy of the extract. The results revealed a dose-dependent reduction in blood pressure levels in rats treated with the extract, with a significant decrease observed in systolic and diastolic blood pressure compared to the control group. Furthermore, the extract exhibited no significant adverse effects on lipid profile or renal function, indicating its potential safety for therapeutic use. These findings suggest that the methanolic extract of Operculina turpethum Linn Silva Manso stem bark possesses promising anti-hypertensive properties, warranting further exploration of its therapeutic potential for the management of hypertension.