AIM- The aim of the present investigation is to study the anti-oxidant activity of various extracts of Punarnava root. MATERIAL METHODS- Dried plant material was powdered and it was packed and sealed in pouch made from cotton fabric and placed in thimble. 0.5 ml of Folin-Ciocalteau reagent and 5 ml of distilled water were combined with a volume of 1 ml from the extract solution, which had been prepared at concentrations of either 1 mg/ml or 0.1 mg/ml. In the determination of total flavonoid content, the aluminum chloride colorimetric assay was employed. The assessment of free radical scavenging activity in bioactive extracts was conducted using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. In the ABTS assay, a 7 mM solution of ABTS was prepared, and its radical cation (ABTS+•) was generated by reacting it with 2.45 mM potassium persulfate, followed by a 12–16 hour incubation in darkness. RESULTS- The extracts of BF were tested for presence and/or absence various phytochemicals like alkaloids, carbohydrates, glycosides, phenolic compounds, flavonoids etc. The content of phenolic compounds was determined from the regression equation of a calibration curve (y=0.011x±0.011) expressed as mg of the both Equivalents (GAE) per mg of extract. The content of flavonoids was determined from the regression equation of a calibration curve (y = 0.1408x + 0.0822, R2 = 0.9983) expressed as mg of Rutin Equivalents (RE) per gram of extract. CONCLUSION- The research on the pharmacological evaluation of indigenous medicinal plants for neuropathic pain represents a significant and promising endeavour that holds the potential to address the limitations of current therapeutic approaches.