Tinosporacordifolia, (TC) sometimes referred to as Guduchi in technical terms, is a key component of Indian medicine. Since ancient times, it has been utilized as a medicine and is a member of the Menispermaceae family. Other names for it are Giloy, Amrita, and Indian bitter. It can be found all over India in deep, dry woods, and at higher altitudes, it develops into small trees and shrubs. TC leaves, root, and stem are all medicinally useful.TC is described as a treatment for a variety of diseases in the classic textbooks of ancient Ayurveda, such as Charaka Samhita, Sushruta Samhita, and other literature, under a variety of names. Tinospora cordifoliaare medicinal plants that have long been used in Ayurveda for a variety of purposes, including Rasayana, Ayushprada, Vayah-Sthapana, Cakshuya, Varnya, Keshya, Vatapitajit, Raktaprasadana, Varnahar, Shothhar, Vishghan, and Chhardighan. However limited informations are available regarding Tinospora cordifolia cytotoxicity potential.The objective of this study was to determine the antiproliferative activity of Ethanolic extract of Tinospora cordifolia(TCE) on MCF-7 breast cancer cell line. Viability of cell was measured using the MTT assay while cell morphology was captured using phase contrast microscope. Results obtained showed that the cell viability decreased in a dose dependent manner TCE similar to the result seen when tamoxifen, an established human anticancer drug is being used. It has been observed IC50 values for ethanol 5.0 ug/ml. Furthermore, cell treated which Tinospora cordifolia extracts detected to be shrunken and detached compared to the untreated cell after 72 hours incubation period. As conclusion, TC has a dose dependent antiproliferative activity against MCF-7 breast cancer cells and the lowest IC50 was seen in ethanol extract. Further studies are needed to elucidate the mechanism of cell death induced byTC.