Rice bran oil contains a wealth of nutritionally significant phytoceuticals like oryzanol, tocotrienols, tocopherols, and other compounds, rice bran oil stands out among other edible oils. One of the ingredients in rice bran oil, oryzanol, has prospective applications in the pharmaceutical, cosmeceutical, and nutraceutical industries. It functions as a natural antioxidant in rice bran oil, where it is found at a concentration of 1% to 2%. In the current investigation, γ-oryzanol was extracted from rice bran oil in a manner that ultimately produced a high yield product. In order to create soap stock, rice bran oil was first treated with alkali and then treated with acid. Following this, the extracted product was washed with distilled water before being crystallized even more. The product was passed through crystallization solvents and then incubated at 4°C in a deep freezer to achieve crystallization. These procedures produced pure γ-oryzanol. The quantization of γ-oryzanol in its crystalline state revealed a concentration of 36.2% at 315 nm. γ-oryzanol is a component of several types of ferulic esters and phytosterols. The only thought to justify the use of γ-oryzanol in a variety of applications, such decreasing cholesterol levels in the human body, is its antioxidant capacity. Using three distinct methods—the DPPH scavenging assay, the nitric oxide scavenging assay, and the reducing power assay—this antioxidant activity was demonstrated in the current work. The findings demonstrated that γ-oryzanol has extremely high antioxidant activity, suggesting that it might be included in a person's diet to help prevent a variety of disorders like hypercholesterolemia and arteriosclerosis that are brought on by excessive cholesterol.