Background:Dialysis is a type of renal replacement therapy wherein the artificial apparatus that eliminates extra water, solutes, & toxins supplements the kidneys' function of filtering the blood. Hence; the present study was conducted for evaluating growth of micro-organisms found in dialysis fluid. Materials & methods: A total of 234 samples were enrolled. Sample fluid was collected after a fixed periodic interval of six days from Dialyzer machines used for the patients undergoing dialysis in the: National Institute of Medical Sciences and Research Jaipur, Rajasthan Desirable Sample volumes were collected for both purified water and ultrapure dialysis fluid. All the results were recorded in Microsoft excel sheet and were subjected to statistical analysis using SPSS software.