This study aimed at Evaluation of hepatoprotective activity in natural plants. In this study Ethanolic extract of powder of plant Dalbergia Sissoo, Hibiscus Rosa and hydroalcoholic extract of Quisqualis Indica was prepared. Albino Wistar rats selected as animal for this study. Extracted sample was used for the hepatoprotective activity in CCI4 induced hepatotoxicity in rats. Various biochemical parameters as SGPT, SGOT, Total Bilirubin, Total Protein, Cholesterol were estimated and histopathological studies were also performed in albino Wistar rat’s liver. The combined extract of all test sample was found to be more significant than separately in CCl4 induced hepatotoxicity in albino Wistar rats. Silymarin used as a standard drug for the hepatoprotective agent in CCl4 induced hepatotoxicity in rats. The results of the present study show that ethanolic extract of Dalbergia Sissoo was more effective than Hibiscus rosa and Quisqualis Indica and combined test sample extract of Dalbergia Sissoo, Hibiscus Rosa and Quisqualis Indica has significant hepatoprotective activity than separate extract.