Background: Bromelain, identified as a cysteine protease enzyme, is widely known for its role in aiding the digestion process by breaking down protein molecules. Extensive research has explored its potential health benefits, including chemoprotection, promotion of wound healing, systemic anti-inflammatory properties, and anticancer effects. Recently, Bromelain has attracted attention for its notable neuroprotective effects. However, additional studies are required to validate its neuroprotective potential. The current investigation focuses on examining the antioxidant capacity of Bromelain, given the crucial role of oxidative stress in the onset of Alzheimer's disease. Moreover, the study assesses the neuroprotective efficacy of Bromelain by examining its impact on acetylcholinesterase enzyme and amyloid beta peptide. Materials and Methods: The free radical scavenging and antioxidant effect of Bromelain was evaluated using in vitro DPPH radical scavenging activity, ABTS radical scavenging activity and Xanthine oxidase inhibitory activity. The effect of Bromelain on acetylcholinesterase activity was measured under in vitro conditions. The inhibitory potential of Bromelain against synthetic monomer amyloid  peptide was also evaluated to confirm the neuroprotective activity exhibited by Bromelain. Results: The free radical scavenging ability of Bromelain was carried out and compared with ascorbic acid. Bromelain has demonstrated significant quenching of both DPPH and ABTS free radicals. Xanthine oxidase, a major source of reactive oxygen species was also found to be markedly inhibited by Bromelain. The in vitro enzyme based acetylcholinesterase activity showed that Bromelain considerably inhibited acetylcholinesterase activity. The Aβ1–42 in vitro results showed that Bromelain effectively inhibited the aggregation of amyloid beta plaques. Conclusion: In summary, the results of the present study showed that Bromelain exhibited significant antioxidant and neuroprotective effect which contributes new insights about the compound.