Research has shown that diode lasers are one of the most commonly used soft tissue laser due to its additional advantages and are nowadays the choice for many periodontal surgeries. Aim: The aim of our study is to assess and compare clinically and microbiologically the treatment outcomes of diode laser alone, open flap debridement (OFD) alone and OFD with diode laser as an adjunct in patients with chronic periodontitis. Settings and Design: This is a prospective, comparative and split mouth study in which lottery method was used for allocation of samples into groups. Methods and Material: Patients were randomly selected and divided into three groups with 15 patients in each group. SRP was performed followed by diode laser pocket decontamination alone, OFD alone and combination of diode laser and OFD in group A, B and C respectively. Assessment of plaque index (PI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL) and healing index (HI) and was done at baseline and after 3 and 6 months. Microbiological assessment of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia was done by culture technique at baseline, immediate, 3 and 6 months postoperatively. Statistical analysis: The statistical analysis was performed using ANOVA, Kruskal Wallis-H test, and Bonferroni test. Results: The differences in clinical parameters in all the groups reached a statistical significance except PI. Significant reduction was observed in colony forming units (CFU) in all the groups but greatest reduction was observed in the combination group. Conclusion: All the treatment modalities were effective in improving clinical and microbiological parameters, although the combination group was more effective.