Due to screening for phytochemicals that may slow or delay glucose absorption, there is great interest in discovering alternative therapies for type 2 diabetes. By showing in vitro evidence of possible inhibition of alpha-glucosidase and alpha-amylase enzymes and then conducting confirming in vivo research on rats, the current work attempts to bolster the biochemical case for more investigation. By suppressing alpha-glucosidase and alpha-amylase, two enzymes that break down carbs, patients with type 2 diabetes and those at risk of the condition may better control their blood glucose levels. Objective: Alpha-Glucosidase and Alpha-Amylase Enzyme Inhibition Research conducted in vitro. Methodology: The necessary bark and roots are collected from their native habitats on the slopes of Yelagiri and Jolarpettai. The pharmacognosy department of Siddha Central Research Institute, Chennai-106 is verifying the raw medications. Use the mentioned amounts after grinding these healing herbs to a coarse powder. Six grams of powder should be added to 240 milliliters of water, which should then be brought to a boil and reduced to 60 milliliters. (1/4) Dosage: 60 milliliters BDS (taken in the morning and evening on an empty stomach) Results: With an IC50 value of 525.5 ± 100 μg/ml, the study shows that the MKC formulation effectively inhibits the alpha-amylase enzyme, with a peak inhibition of around 52.42%. At the same time, the alpha-glucosidase enzyme was significantly suppressed by traditional acarbose, with an IC50 value of 33.17 ± 17 μg/ml and a maximal inhibition of around 98.47 ± 1.86%. Conclusion: According to the study's findings, the MKC formulation significantly inhibited the Glucosidase enzyme, with an IC50 of 591.1 ± 232.2 μg/ml and a maximal inhibition of around 46.83 ± 15.95%. In contrast, the alpha-amylase enzyme activity was significantly reduced by conventional acarbose, with a maximal inhibition of 99.16 ± 0.3852% and an IC50 value of 24.97 ± 5.389 μg/ml.