Nigella sativa (Family Ranunculaceae) is one of the medicinal plant species that has become well-known for a variety of medical uses due to its rich source of phytoconstituents. Ongoing scientific research on N. sativa seeds is necessary to have a deeper comprehension of its numerous medical applications. The present study is focused on analyzing the phytochemicals present in the Nigella sativa (NS)seeds, and its anti-oxidant and anti-microbial activity. Crude extract of Nigella sativa of solvents extracts (acetone, petroleum ether, chloroform, diethyl ether, hexane, and ethanol) were analyzed for their phytochemical properties by using standard methods. Based on the qualitative phytochemicals profile, ethanol, acetone, petroleum ether, and hexane solvent extracts were analyzed for their anti-oxidant properties by DPPH assay and antimicrobial activity against human pathogenic organisms (Staphylococcus aureus, E.coli, Pseudomonas auroginosa and Enterobacter sps)by well diffusion method. Results of phytochemical studies reveal the presence of terpenoids, saponins, alkaloids, flavonoids, carbohydrates, steroids, phytosterol, cardiac glycosides, anthraquinones, polyphenol but glycosides were absent in all six solvent extracts. The antioxidant activity of selected solvent extracts shows close anti-oxidant properties to reference compounds when using higher concentrations of extracts. Each solvent extract shows potential inhibitory activity against the selected pathogenic microorganism. Therefore, this study confirms that N.sativa seeds extract contains a variety of chemical components and active functional groups linked to their antioxidant properties and antimicrobial activity.