Vitamin D deficiency and anemia are important public health problems and are common in both acute and chronic illness. so this study aimed to determine vitamin D and iron profile levels among anemic and apparently healthy Sudanese individual in Khartoum State. This descriptive cross sectional study was conducted to assess vitamin D and iron profile among 60 patients with anemia, they were 20 (33.3%) males and 40 (66.7%) females and 30 apparently healthy individual they were 14 (46.7%) males and 16 (53.3%) females. in this study There were 34 patients with iron deficiency anemia (79% of whom had vitamin D deficient), while 21patients with anemia of chronic disease (71% had vitamin D deficient), also there were 5 patients with megaloblastic anemia (60% had vitamin D deficient) and 30 apparently healthy individual (70% had vitamin D deficient). The vitamin D level should no significant difference between anemic patients and apparently healthy individual (P V > 0.05), so vitamin D deficiency and insufficiency was prevalent, in all the groups. This study concluded that there was very low level of vitamin d among anemic and apparently healthy individual. Regarding iron profile also there was different variance in the results that indicate apparently healthy individuals have some types of pre-latent and latent iron deficiency anemia. As for the relationship of vitamin D with iron profile, there was no correlation between iron profile and vitamin D level among anemic and apparently healthy individual. with regard to the level of vitamin D between both sexes, there was a significant decrease in the level of vitamin D in females compared to meals.