According to the World Health Organization (WHO), the prevalence rate of superficial mycotic infection worldwide has been found to be 20-25%. Medicinal components from plants play an important role in conventional as well as western medicine in treating fungal diseases. Plant derived compounds as potent drugs have been a part of human evolution and healthcare for thousands of years. Main aim of this study is to analyze the zone of inhibition produced by homeopathic medicine in various potencies in the culture media proving its antifungal activity. The medicine Kalmia Latifolia 30C, 200C and 100 is used for the study. Fungi Aspergillus Niger, Aspergillus Flavus and Candida Albicans were purchased from Microbial Type Culture Collection and Gene Bank (MTCC) Chandigarh. The bacterial strains were maintained on Nutrient Agar (NA). Antibiotic susceptibility tests were determined by agar disc diffusion (Kirby-Bauer) method. Kalmia 200C shows maximum effectiveness against the fungal strains