The aim of our present study was to explore the Antioxidant and Antidiabetic potential of Mutingia calabura fruit extract by invitro analysis. Methods: Muntingia calabura fruits were clean dried, powered, sieved, soaked and kept for 2hr in ethanol and simultaneously aqueous extract was prepared. The samples were filtered and utilized for further analysis. Ferric reducing antioxidant power (FRAP) and nitric oxide radical scavenging activity was assayed to assess the antioxidant activity of Mutingia calabura fruit extract. In addition to assess the Antidiabetic activity, α-amylase and α-glucosidase inhibitory activity was measured, hydrolysis index and glycemic index were also assessed. Results: The aqueous and ethanolic extract of Muntingia calabura fruit extract shows a very good free radical scavenging activity in a dose dependent manner. In addition to this the extracts were showed a gradual increase in the inhibition of both α-amylase and α-glucosidase in a dose dependent manner. It also showed a low glycemic index, hence it may play a vital role in preventing or managing diabetes mellitus.