The herbs plant based medicine system utilized for various diseases treatment in Ayurveda medicine for many decades due to its great and broad medicinal values. The present study aimed at carrying out phytochemical analysis and evaluates its in-vitro antidiabetic activities of traditional medicinal plant Glycyrrhiza glabra. The plant root powder was collected from the local site of Davangere city, Karnataka, India. The Extraction of plant material was followed by the cold extraction method. An in vitro antidiabetic activity was evaluated by alpha-amylase method and α–Glucosidase inhibition assay method and estimated the rate of glucose uptake using 3T3L-1 cells. The Methanolic extracts of Glycyrrhiza glabra root showed significant inhibition of alpha-amylase and Alpha Glucosidase inhibition activity as compared with standard drug Acarbose and IC50 value was calculated. The phytochemical analysis revealed that Methanolic extract of Glycyrrhiza glabra root showed presence of secondary metabolites Alkaloid, Tannin, Terpenoids, Steroid, Saponin, Flavonoids, Mucilage, Volatile oil and these different phyto-compounds which might be responsible for significant in vitro antidiabetic activity. Further identification and isolation of bioactive substances and secondary metabolites from such medicinal plants and there in vivo efficacy evaluation will help in further validation of pharmacological activities in future prospective days.