This research explores the morphological, metabolic, and biochemical characteristics of Streptomyces isolates from various soil types in Kogi Central, Nigeria, and their potential antimicrobial and antiulcer activities. Isolates were collected from grass, yam, cassava cashew farm and refuse dump environments . Morphological examination revealed diverse features: grassland isolates (GA 1, GA 2, GA 9) displayed varying appearances and spore arrangements, while isolates from yam farms (YM 1, YM 2, YM 3, YM 4) and cassava farms (CSV 1, CSV 2, CSV 3) also exhibited distinct pigmentation and texture patterns. Refuse dump isolates (DM 1, DM 2, DM 3, DM 4) and cashew farm isolates (CW 1, CW 2) showed unique pigmentations and surface textures. Sugar utilization studies indicated metabolic diversity, with isolates from different environments demonstrating varied abilities to metabolize sugars, suggesting adaptability to diverse ecological niches. Antimicrobial assays showed significant activity against ulcer-associated pathogens. For instance, isolate GA 1 exhibited strong inhibition against Helicobacter pylori and Escherichia coli, while GA 9 was particularly effective against Salmonella spp. The antiulcer activity of these isolates was also assessed using various ulcer models. Isolates GA 1, GA 2, and GA 9 showed substantial efficacy in mitigating aspirin-induced, pylorus ligation, ethanol-induced, and cysteamine-induced ulcers, with GA 1 demonstrating the highest overall efficacy. These findings show the potential of Streptomyces isolates as sources of novel antimicrobial and antiulcer agents. The morphological and biochemical diversity observed suggests significant ecological and practical relevance.