ISSN : 2663-2187

EXTRACTION, CHARACTERIZATION AND PHYTOCHEMICAL ANALYSIS OF EXTRACT OF DOLICHANDRONE FALCATA PLANT

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Sagar Kamble, Vishal Pande, Nagesh Tour, Yogesh Mahajan
ยป doi: 10.33472/AFJBS.6.9.2024.516-528

Abstract

This study conducts a thorough investigation of the plant Dolichandrone falcata using a complex procedure involving extraction, characterisation, and analysis of its phytochemicals. The study thoroughly examines the composition and features of the plant, use a range of procedures to assure precision and dependability. The investigation commences with thorough botanical, macroscopic, and microscopic exams, meticulously recorded with pictures and photographs. Microscopic examination is an essential method for examining contaminants, guaranteeing the cleanliness and genuineness of the plant material being researched. The physicochemical analysis confirms the validity and quality of the plant material through the assessment of various characteristics. These criteria include the presence of foreign organic matter (0.45% w/w), compliance with moisture content, and the determination of ash values (total ash: 2.8%, acid insoluble ash: 0.34%, water soluble ash: 0.11%). The plant's extractive values demonstrate its potential for pharmacological action and extraction efficiency, with a higher ethanol-soluble extractive value of 14% w/w compared to a water-soluble extractive value of 3.8% w/w. The process of continuous hot Soxhlet extraction was used to get extracts in methanol (8.3%), ethanol (18.1%), and chloroform (5.5%). Prior to extraction, a preliminary phytochemical screening was conducted. The phytochemical screening of these extracts indicates the presence of vital components like alkaloids, glycosides, flavonoids, and terpenoids. The ethanol extract is particularly noteworthy due to its substantial metabolites, suggesting its potential for additional investigation. The bioactive ethanol extract is thoroughly characterised using Thin Layer Chromatography (TLC). Specific mobile phases and detection technologies are utilised to visualise and identify various chemical elements, such as alkaloids, glycosides, flavonoids, tannins, and terpenoids. The measured Rf values offer crucial information for the isolation and identification of specific bioactive chemicals

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