Bio polymers are polymerized high molecular constituents from natural substances from the natural substances by the chemical or biological reactions. The most common biopolymers are polynucleotides, polyamides, polysaccharides, polyoxoesters, polythioesters, polyanhydrides, polyisoprenoides polyphenols, and their derivatives. Among the polysaccharides the starch, chitin, and cellulose are widely used biopolymers from the plant source. In this current study cellulose was extracted from oil industry waste such as sesame oil cake (Sesamum indicum), peanut oil cake (Archishypoogaea), andcoconut oil cake (Cocus nucifera) and the plant samples ofpalm (palmae) and taro (Colocasia esculenta). The extracted crude cellulose was estimated by dry weight, and UV-spectrophotometric method. The functional properties are characterized using Fourier Transform Infrared (FTIR) analysis. Three different sample of palm leaves, sesame oil cake and coconut oil cake yielded the better quantity of 18.14 g (w/w), 10.78g and 9.80 g in dry weight analysis than the other samples. Hence, the spectrophotometric quantification resulted the cellulose content of 0.25g/100g in palm leaves, 0.17 g/100g in sesame and coconut oil cake samples. The FTIR analysis also confirms that the structural properties of cellulose in these samples. Hence, the developed biodegradable film from palm leaves, sesame have the better morphology in SEM analysis. Therefore, the study results conclude the palm leaves and sesame oil cake were the suitable raw material for extraction and development of cellulose based biodegradable film.