Introduction: Guided tissue regeneration (GTR) treatment of periodontitis is a surgical procedure to implant a regeneration membrane that specifically aims to restore the periodontal tissues supporting the teeth. Electrospinning was applied to fabricate the Polyvinyl alcohol membrane and to graft chondroitin sulphate on its surface. Chondroitin sulfate is a major component of many connective tissues, including cartilage, bone, skin, ligaments and tendons. The Cissusquadrangularis plant has been used to treat a variety of ailments. Few studies suggest that it may have medicinal properties like maintenance of bone health. The current study aims to prepare a GTR membrane composed of polyvinyl alcohol, chondroitin sulphate, cissusquadrangularis and extracellular matrix and to check the properties of the novel membrane.