INTRODUCTION: Guided tissue regeneration (GTR) prevents connective tissue from entering the bone reformation site and interference in osteogenesis. It also creates a space under the surgical flap that acts as a scaffold for the growth of cells and blood vessels. This study aims to develop a novel electrospun scaffold by incorporating bone powder into Cissusquadrangularis extract and PVA, harnessing the synergistic properties of these constituents for enhanced tissue regeneration. The membrane's design aims to achieve optimal porosity, mechanical strength, and bioactivity to foster guided tissue regeneration in clinical applications.