In this study, we successfully prepared a Boerhavia diffusa extract-loaded nanofiber wound dressing using the electrospinning method, optimizing the process through a 3x2 factorial design. The optimized nanofiber, denoted as BRNF7, exhibited excellent characteristics, including an entrapment efficiency of 85.12% and a drug loading of 9%. Furthermore, the swelling index was measured at 86.6, demonstrating its potential as an effective wound dressing material. Characterization of the BRNF7 nanofiber revealed promising results, as FTIR spectra exhibited all the expected peaks, while XRD analysis indicated a highly crystalline structure with intense peaks. DSC thermograms displayed a characteristic peak at 133.42°C, corresponding to Boerhavia diffusa extract. Thermal analysis, as indicated by TGA thermograms, showed that the nanofiber gradually decomposed at a higher temperature range, signifying its thermal stability. Wound dressing various parameter included as the film thickness ranged from 0.069 to 0.074 mm, and all films exhibited excellent folding endurance, ensuring flexibility. Moisture interaction studies demonstrated good physical stability of the film under high humidity conditions and maintained integrity under dry conditions. The films exhibited a moisture absorption range of 3.8% to 4.6% and a moisture loss range of 3.2% to 4.7%. Tensile strength values ranged from 2.14 to 2.85 kg/cm², and the percentage of drug content was uniform between 90.15% and 92.11%.Furthermore, our Boerhavia diffusa extract-loaded Nanofibrous dressing displayed notable antibacterial activity against various pathogens and exhibited promising wound-healing effects, making it a potential candidate for advanced wound care. This study provides a comprehensive assessment of the BRNF7 nanofiber, emphasizing its potential in biomedical applications