This study aimed to evaluate the phytochemical composition and antioxidant potential of a plant extract through various assays. Phytochemical qualitative analysis revealed the presence of anthraquinones, tannins, saponins, flavonoids, glycosides, terpenoids, and steroids. Further quantitative assays determined the total flavonoid fraction and total glycoside content in the extract. Additionally, the DPPH radical scavenging activity assay was conducted to assess its antioxidant capacity. Results indicated significant levels of flavonoids and glycosides in the extract, with total concentrations of 2036.2 µg and 861.9 µg, respectively. The DPPH assay revealed concentration-dependent scavenging activity, with the extract demonstrating substantial inhibition of DPPH radicals, comparable to reference antioxidants Quercetin and Vitamin C at higher concentrations. These findings suggested the potential therapeutic value of the extract as a natural antioxidant agent.