AIM: To assess the anti-inflammatory, antioxidant, antimicrobial and cytotoxicity properties of the formulated mouth rinse. MATERIALS AND METHODS: In this study, the mouth rinse formulation was subjected to anti-inflammatory, anti oxidant, antimicrobial and cytotoxicity testing RESULTS: Highest anti-inflammatory and antioxidant properties were noted at 50 μl concentration when compared to the control. The antimicrobial activity of the kelp mouth rinse had the highest zone of inhibition seen in 80 μL concentration. The cytotoxic activity was found to be better at all concentrations studied. CONCLUSION: The formulated mouth rinse exhibited good anti-inflammatory, antioxidant and anti microbial activities and showed low cytotoxicity activity.