In the present study, we have developed herbal hair dye. Most of the marketed synthetic hair dye formulations contain paraphenylenediamine at 20-25% concentrations. It is known to trigger allergic skin reactions in many people. Hence, there is need of herbal based hair dye which will not contain any harmful chemical constituents and which gives colours to hair. In this study, herbal hair dye containing Annona squamosa and Nigella sativa as a chief constituent was used. As Annona squamosa is a great natural source of copper and gives hair the dark melanin colour and that it lack. It also helps to deal with premature greying of hair in both men and women. Nigella sativa seed has antimicrobial, antioxidant, anti-aging, hair growth promoter, hair dying, sun protection, anti-cancer activity, which make it a novel ingredient for many cosmetic preparations. Other drugs used are Henna, Indigo, Bhringraj, Wallnut hull, Coffee, Amla, Shikakai, Hibiscus etc. for the preparation of herbal hair dye. Totally, 27 formulations were prepared using MINITAB software with factorial design of 5^3. The formulated herbal hair dye was evaluated for organoleptic, physicochemical, phyto-chemical, rheological, patch test, stability test, dye test and colour retention capacity. It was found that herbal preparations is greenish in appearance with characteristic odour and having a fine texture. The pH was 6.7 which is normal to the human scalp and hairs. The rheological parameters were in a acceptable range. All the formulations were found to be a good dying efficacy and colour retention capacity with no irritancy, swelling or redness. The formulation F25 showed highest colour retention capacity in comparison with marketed herbal preparation upto 50 Vs 32 days respectively. The formulated formulation is stable at room and at 40oC temperature