This study investigates the optical characteristics and calibration information Fluticasone propionate in various solvents, focusing specifically on methanol and phosphate buffer (PBS) with the addition of Tween 80. Concentrations ranging from 5 to 40 µg/ml were used to produce calibration curves. The average absorbance, together with the corresponding standard deviations, in the methanol calibration data exhibited properties of linearity and repeatability. In addition, the high regression coefficient (R²) of 0.999 provides additional evidence of the great conformity to Beer-Lambert's rule. The PBS-Tween 80 solution had a high degree of linearity, as shown by its R² value of 0.999, confirming its consistency when tested with different solvents. In addition, the pre-formulation research of Fluticasone propionate (FP) provided insights into its physicochemical properties and its compatibility with various excipients, showing no significant interactions under normal conditions. Through the use of pseudo-ternary phase diagrams and experimental methodologies, the study aims to improve the solubility and stability of the components in order to develop a microemulsion formulation for FP. The solubility and globule size of the optimised formulation were both at their optimum levels, resulting in a considerable enhancement in the potential of FP for topical application. The results highlight the reliability of the analytical methods used to calibrate Fluticasone propionate and the effective formulation approach utilised for FP Microemulsions. These findings provide valuable insights for prospective pharmaceutical applications in the future.