The present research has been undertaken with the aim to formulate and evaluate the herbal cream containing ethanolic flower extract of Blumea lacera (Burm.f.) DC., Leucas cephalotes Roxb. and Peristrophe bicalyculata (L) Merr. The poly herbal cream was prepared using ethanolic flower extract in varied concentrations and was evaluated using physiological measurements. The polyherbal cream was prepared by using bees wax, paraffin wax, olive oil, soya powder, tocopherol, glycerine, rose water, peppermint oil and sandalwood oil and required amount of distilled water. Then skin pH (6.8-7) was maintained by drop wise addition of tri-ethanolamine. The physiochemical parameters of formulations (pH, viscosity, spreadability etc.) were determined. The results showed that formulation PHC-III have maximum drug content and drug release. Further PHC-III was screened for anti-fungal activity against Candida albicans, Cryptococcus neoformans and Aspergillus flavus. The zone of inhibition was recorded and anti-fungal activities were determined and results were compared with standard marketed herbal cream.