This study investigates the impact of prolonged starvation on hepatic glycogen levels in Clarias batrachus, a commonly found air-breathing catfish. Both male and female specimens were utilized due to their widespread availability in local ponds and their ability to endure extended periods of starvation. The glycogen content in the liver was assessed after 0, 10, 20, 30, and 40 days of starvation to evaluate the effects of fasting on hepatic tissues. Glycogen levels were determined using a modified version of the calorimetric method developed by Kemp et al. (1954) and Krishnaswamy & Srinivasan (1961). Our results indicate a gradual depletion of glycogen in the liver over the course of starvation. Interestingly, females exhibited higher levels of glycogen compared to males under both normal feeding and starved conditions. However, after 40 days of starvation, males demonstrated a more pronounced depletion of liver glycogen compared to females. These findings shed light on gender disparities in energy metabolism during prolonged periods of fasting in Clarias batrachus.