Antimicrobial resistance, abbreviated as AMR, has arisen as a worldwide health risk, contributing to higher rates of morbidity, death, and overall expenses associated with medical treatment. Within the context of this multi-center study, the purpose was to assess the efficacy of treatments using genotypic monitoring and antibiotic stewardship in the fight against antimicrobial resistance (AMR) in hospital settings. In order to evaluate the effect that these measures have had on the development of antibiotic resistance patterns, an exhaustive study of patient records and bacterial isolates from a number of different hospitals was carried out. Our research sheds light on the important part that genotypic surveillance and targeted antibiotic stewardship methods play in preventing the spread of antibiotic-resistant bacteria and increasing the efficiency with which antimicrobials are utilised