Elicitation seems to be a promising alternative to other conventional biotechnological techniques used for improving bioactive compounds and biological activities of sprouts. The purpose of this study was to evaluate the effect of amino acids as elicitors such as glutamic acid (1 mM, 5 mM, 10 mM) and L-phenylalanine (0.1 mM, 0.5 mM, 1.0 mM) on oil content, nutritional parameters such as ascorbic acid and beta-carotene; and anti-nutritional parameters such as saponin and phytic acid for soybean sprouts. The results revealed that maximum recovery of oil content in soybean sprouts were obtained at 1 mM glutamic acid (24.40%). The effect of elicitation on ascorbic acid and beta-carotene during the sprouting of soybean was found highest at 1.0 mM L-phenylalanine elicitor as 54.42 mg/100g and 4.88 μg/g respectively. The anti-nutritional parameters such as saponin and phytic acid were reduced after the treatment of elicitor during the sprouting process in soybean sprouts. The lowest values of saponin content and phytic acid were examined as 3.27 mg/g and 1.34 mg/g at the highest concentration of L-phenylalanine and glutamic acid respectively. The use of these amino acids as chemical elicitors significantly improves the quality of soybean sprouts.