This experiment was conducted out in Al- Suwaira farms in Wasit during the winter season of 2021-2022.To examine the effects of nano gold at concentrations of (0, 25, 50, 75, and 100) ppm and nano copper at concentrations of (0, 10, 20, 30, and 40) mg/L on germination percentage, vigor index I VI (I), 1000 seeds weight, carotenoids, volatile oil, anise-aldehyde, anise on growth single and two way interaction. Just one application of the previously listed compounds was shown to have an important impact on anise seed development and plant physiology, particularly at high concentrations. The effective use of two-way interactions resulted in a variety of consequences. In a completely randomized design, treatments are developed as a perfectly randomized factorial experiment (5×5×3), with three replicates. The aim of this research is to determine anise with (gold and copper) nanoparticles effects on seed growth and plant physiology.