The brain and spinal cord are connected to all body’s organs and to the outside world by the peripheral nervous system that has a restricted capability to regenerate. Fibrin glue (FG) can be used as an alternative to suture repair to reconnect peripheral nerves. Aim: This study aims to compare the use of proline suture and FG in nerve repair through histological examination and immunohistochemical detection of PCNA. Materials and Methods: 24 albino rats divided into 3 groups GI: subjected to 3 hours of compression with a non-traumatic vascular clamp on left sciatic nerve then clamps were removed, GII: subjected to immediate repair of the nerve stumps by direct proline suture while GIII: subjected to repair with FG adhesion. After eight weeks, the nerves were removed and prepared for histological and immunohistochemical studies. Results: GI showed multiple destructive histopathological results while in GII and GIII the neural tissue was almost restored to normal reported with significant increase in PCNA positive cells. Conclusion: regeneration of nerve injury was reported for both treated groups with some privilege to FG in repairing transected sciatic nerve.