Aim: Histomorphological Analysis of Endoscopic Biopsies in Upper Gastrointestinal Tract Lesions. Material and Methods: This prospective study was conducted on 100 patients who underwent endoscopic biopsies for the evaluation of upper gastrointestinal tract (GIT) lesions. The biopsy samples were collected from patients of all age groups and both sexes who met the inclusion criteria. Endoscopic biopsies were taken from the margins of the lesions by specialists using standard endoscopic techniques. The biopsy specimens were fixed in 10% formalin and then sent to the pathology department, accompanied by the endoscopic findings for histopathological analysis. Upon receiving the samples, the tissue specimens were processed and embedded in paraffin. Thin sections, approximately 4 microns thick, were cut from the paraffin-embedded tissues and stained with hematoxylin and eosin (H&E) for routine histological examination. In cases where further diagnostic clarity was needed, special stains such as modified Giemsa for Helicobacter pylori detection and periodic acid-Schiff (PAS) for identifying intestinal metaplasia were employed.