Taking into account the controversial nature of data pertaining to plasma arginine levels in hypertension, the fact that humans only synthesize NO from this amino acid, the crucial role that NO plays in preserving endothelial function, and the significance of microalbuminuria early identification. Aim: Thus, this study set out to determine if hypertension patients with microalbuminuria had elevated levels of nitric oxide reactive species (NOS) and serum arginine, and if so, whether or not there was a link between these two variables and urine microalbumin. Materials & methods: The S.C.B. medical inpatient and outpatient departments will be closed from October 2018 until September 2019. Thirty-one hypertension patients without microalbuminuria attended the Medical College in Cuttack, Odisha, India; the patients’ count was fifty- one. Thirty-one healthy volunteers, matched for age and sex, made up the control group. This research was carried out in collaboration with the departments of medicine and biochemistry at the S.C.B. Medical College in Cuttack, which is located in the central Indian state of Odisha. Results: The study examined the Pearson's association between NO (μmol/L) and arginine (ng/ml) in individuals with hypertension. The results showed a substantial positive connection (p = 0.014, r = 0.612). In hypertensive individuals, the Pearson's correlation between urine albumin (mg/dl) and arginine (ng/ml) was examined. With an r-value of -0.364 and a p-value of 0.001, it was discovered that there was a significant inverse association occurring. Conclusion: In this part of India, hardly much research has been done on arginine. The results of the present study indicated that arginine and nitric levels were positively correlated with critical hypertension. Nevertheless, larger-scale, multicenter studies may be worthwhile to investigate arginine's therapeutic potential in the setting of hypertension.