Putranjiva roxburghii wall. is a valuable medicinal plant used in conventional therapeutic system to treat several diseases. It is an evergreen tree of tropical Asia, used in all system of ethno-pharmaceutical practices adopted in Asian countries. Phenolic compounds and flavonoids are the major metabolites reported in leaf, stem and root of this plant and are established to be associated with its medicinal importance. This study demonstrates that Principal Component Analysis (PCA) can be effectively used for identification and discrimination among major medicinal metabolites extracted with three different solvents, present in different plant parts of Putranjiva roxburghii.