Faba bean (Vicia faba L.) plays a crucial role in global food security and sustainable agriculture. However, its cultivation faces challenges due to various diseases that can significantly impact crop yield and quality. This study focuses on exploring the multifaceted aspects of disease resistance in faba beans, with a particular emphasis on developing cultivars with strong defense mechanisms against multiple pathogens. This study investigates the resistance of forty-six faba bean (Vicia faba L.) germplasm lines against three major pathogens: root-knot nematode (Meloidogyne incognita race-1), Alternaria blight (Alternaria alternata), and leaf curl virus (family- Begomoviridae). The primary aim was to identify germplasm with significant resistance for potential use in breeding programs. Under controlled conditions, each germplasm line was inoculated with nematode juveniles and assessed for root gall formation. Five varieties (FLIP15-139FB, S2011,134, FLIP19-207FB, L2014-010, HB-19-13) demonstrate resistance/tolerance to root-knot nematode, Alternaria blight, and leaf curl virus. These varieties are highly recommended for commercial production in regions where multiple diseases co-exist, ensuring robust crop performance. These findings underscore the critical importance of diverse germplasm screening to enhance the disease resilience of faba beans, contributing to sustainable agricultural practices.