Background: Post-menopausal women are at a heightened risk of cardiovascular disease (CVD) due to hormonal changes and dyslipidemia, which disrupt lipid metabolism and vascular health. Understanding the relationship between these factors is essential for improving cardiovascular outcomes in this population. The objective was to ‘to investigate the impact of dyslipidemia and hormonal changes on cardiovascular health in post-menopausal women by assessing lipid profiles, inflammatory markers, and subclinical vascular changes’. Methods: This cross-sectional study conducted at Muhammad College of Medicine, Jan 2023 to Jan 2024, included 85 post-menopausal women aged 45 70 years. Participants were assessed for lipid profiles (LDL cholesterol, HDL cholesterol, triglycerides, and total cholesterol), hormonal levels (estradiol and follicle-stimulating hormone), and inflammatory markers (hs-CRP). Cardiovascular health was evaluated using systolic and diastolic blood pressure, carotid intima-media thickness (CIMT), and echocardiographic ejection fraction. ‘Statistical comparisons between groups with and without dyslipidemia were conducted, and p-values <0.05 were considered significant’. Results: Women with dyslipidemia exhibited significantly higher LDL cholesterol, fasting glucose, hs-CRP, and CIMT levels, along with lower HDL cholesterol compared to those without dyslipidemia (p < 0.05). Hormonal changes, particularly reduced estradiol levels, were associated with unfavorable lipid profiles and increased inflammation. Elevated blood pressure and markers of subclinical atherosclerosis further underscored the heightened cardiovascular risk in the dyslipidemic group. Conclusion: This study demonstrates that dyslipidemia, compounded by post menopausal hormonal changes, significantly impacts cardiovascular health through lipid imbalances, systemic inflammation, and subclinical vascular changes. Early detection and management of these risk factors are crucial for reducing cardiovascular morbidity in post-menopausal women.