Pilates exercise training is a form of physical conditioning that focuses on core strength, flexibility, balance, and body control. It was developed by Joseph Pilates in the early 20th century and has gained popularity as a holistic approach to fitness. These exercises are typically performed on mat or using specialized equipment such as the Pilates reformer.The main objective of this Paper is to examine the effect of Pilates exercise on improving strength and performance in football players. 34 football players between the age group of 19-25 who were actively involved in the sports at Mewar University football ground from different parts of globe participated in this research. The study group was divided into two groups as the players were from different countries and continents and as per their genetics some are very prone to get injured. To get the exact effect of Pilates on football players, set of data was collected before applying the training module of Pilates. The next data was collect after successfully completing the 8 weeks’ program. It was observed that there were major statistical changes in the data which motivated me to collect the next set of data after another 10 weeks. The research found that the football players from African continent were more benefited from the Pilates exercise as they are comparatively genetically fit than the Asian players.