The utilization of in vitro cultures for the examination of stress responses depends on the idea that cells cultivated in vitro exhibit comparable behavior to cells in intact plants that are exposed to situations of water deficiency and salinity. The objective of this study was to investigate the physiological responses of date palm, cv. Bartemoda, shoots proliferation and rooting to salinity stress. A small cluster consisting of 3-5 developed shoots were cultured on MS medium supplemented with BA at 2.0 mg/l + TDZ at 0.5 mg/l with the inclusion of various concentrations of NaCl at (0, 500, 1000, 1500, and 2000 ppm) with five replicates. The exposure to salt stress resulted in when used 500 ppm NaCl produced significant increases of the shoot number (9.0 shoot/cluster). The number of shoots decreased from 7.0 to 4.0 shoot/cluster with the increase of NaCl concentrations from 1000 to 2000 ppm. The rooting percentage decreased significantly using 1000 ppm NaCl (65%) and was the lowest at 2000 ppm NaCl (30%). The rooting percentage at 500 ppm NaCl was increased significantly (85%) as compared with the control treatment. For healthy ex vitro growth of date palm plantlets in a greenhouse, the quality of the shoot and root produced in the laboratory is crucial.