The present investigation entitled“Impact of Soil Parameters and Microbial Communities on Bael (Aegle marmelos) cv. NB-9 under Subtropical Conditions”was carried out during the year 2022-2024 at Main Experimental Station, Department of Fruit Science, College of Horticulture & Forestry, A.N.D.U.A.&T., Narendra Nagar (Kumarganj), Ayodhya, Uttar Pradesh, India (Lat 26.540849° Long 81.830421°). Application of various bio-formulations and boric acid with eco-friendly materials to study the differentsoilproperties-soil pH, electrical conductivity,organic carbon, available nitrogen, available phosphorus, available potassiumand microbial population i.e., bacteria, fungus and actinomycetesand effect on the vegetative growth of fruit. The experiment was laid down in randomized block design (RBD) with (12) treatments and (03) replications.The experiment consists of 12 treatments including control, T1 (Control), T2 (FYM 50 Kg), T3 (FYM 100 Kg), T4 (Boric acid 200g), T5 (Boric acid 400g), T6 (Biofertilizer 250g/plant [100g Azotobacter + 100g Biovita + 50g PSB]), T7(FYM 50Kg + Boric acid 200g), T8 (FYM 50Kg + Boric acid 400g), T9 (FYM 50Kg +Biofertilizer 250g/plant [100g Azotobacter + 100g Biovita + 50g PSB]), T10 (FYM 100Kg + Boric acid 200g), T11 (FYM 100Kg + Boric acid 400g) and T12 (FYM 100Kg +Biofertilizer 250g/plant [100g Azotobacter + 100g Biovita + 50g PSB]) was used for this study. The results revealed that treatment T7 and T12 outperformed the rest with minimum soil pH (8.01),electrical conductivity (0.40 dSm-1), fungal population (2.91 105cfu g-1) and maximum organic carbon (0.60 %),available nitrogen (272.14 kg/ha), available phosphorus (19.68 kg/ha), available potassium (360.17 kg/ha),bacterial population (8.05 106cfu g-1), actinomyctes population (7.29 105cfu g-1),was most effective froma vegetative growth point of view. Thus, the treatment combination T7and T12 is recommended for application to Bael trees in subtropical conditions to obtain good physical and chemical characteristics with better quality fruits.