The aim of this study was to evaluate the significance of RBC Scatterogram using an Automated Hematoanalyzer in the diagnosis of Iron deficiency Anemia (IDA) and to see the treatment response in comparison to other conventional iron parameters. MATERIAL AND METHODS - A total of 10 adult patients with Iron Deficiency Anemia (Serum Hemoglobin <11g/L and serum ferritin <15ng/mL ) were enrolled. RESULTS –Scatterogram in IDA patients consisted mostly microcytic RBCs which gradually changed to normocytic RBCs with treatment response. RBC Scatterogram showed a significant positive correlation with Hemoglobin, MCV & RDW. CONCLUSION – Our data demonstrate that RBC Scatterogram is a useful parameter that can be confidently used in the diagnosis of IDA , and also in evaluating its response to treatment