Inflammation is essential for the body's defence mechanism but can lead to health issues when chronic inflammation persists over extended periods. Anemone rivularis Buch.-Ham. ex DC., native to the West Jaintia Hills of Meghalaya, India, is investigated for its anti-inflammatory potential. This study aim to assess the anti-inflammatory property of Anemone rivularis leaf extracts through phytochemical analysis and protein denaturation inhibition assays. Leaves of Anemone rivularis were subjected to maceration and hot percolation extraction technique by using methanol. Phytochemical analysis was done to identify bioactive compounds and egg albumin denaturation assay was conducted to evaluate anti-inflammatory activity. Phytochemical analysis revealed flavonoids, saponins, steroids, triterpenoids, tannins, and phenolic compounds in methanolic extracts. The extracts also exhibited significant inhibition of protein denaturation, indicating potential anti-inflammatory activity. So it can be concluded that Anemone rivularis shows promise as a natural anti-inflammatory agent with an percentage inhibition for standard 55.45% at a concentration 6.25µg/ml and for extract was 88.86% at a concentration of 0.001µg/ml. Further scientific investigation is necessary to confirm its efficacy and safety for preclinical and clinical use, highlighting its potential as a novel therapeutic option for managing inflammation-related conditions.