Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
The emergence of antibiotic-resistant bacteria has revived interest in a large group of plant antimicrobial compounds, such as essential oils, as alternative antimicrobial agents to combat AMR. The essential oils of Dacryodesbuettneri and Dacryodesedulis were obtained by hydrodistillation of the resins, then tested against the bacteria responsible for childhood diarrhea by the disc diffusion method. The MIC was determined by the microdilution method (CLSI). Temporal killing of bacteria was obtained using the standardized CLSI M26-A test. The checkerboard test was used to evaluate the in vitro synergy of essential oils with the antibiotics prescribed to combat bacterial gastroenteritis in Gabon. Essential oils have shown antibacterial activity against high-level penicillinase and ESBL strains. Additionally, D. edulis demonstrated antibiofilm activity against all strains studied. Similarly, E. coli ATCC 25922 and Shigellaspp were sensitive to D. buettneri essential oil. The (CFM)-D. edulis combinationshowed synergy on penicillinase strains, and the (CRO)-D. eduliscombination. against ESBLs of E. coli. Overall, our results showed that both essential oils can be considered as alternatives to antibiotics in the fight against AMR. However, their simultaneous use with antibiotics should be avoided.