Objective: Streptococcus mutans (S. mutans) primarily inhabits biofilms that form on tooth surfaces, also known as dental plaque. S. mutans is a gram positive, non-motile coccus is resistant to most of the conventional antibiotics including rifampicin, ciprofloxacin, and metronidazole. Due to the abundance of various virulence factors found in S. mutans it is recognized as a key pathogen in the formation of dental cavities. So, there is a need of a natural compound which have an imprecise therapeutic value all over the world. This research purpose is to investigate the antimicrobial and antibiofilm activity of the plant extract Saraca indica (S. indica) against S. mutans isolate. Methods: Examined the antimicrobial and antibiofilm activity of natural plant extract of Saraca indica (S. indica) against S. mutans by several in-vitro investigations such as crystal violet biofilm inhibition assay and growth curve. Results: At 2.5mg/mL, methanolic extract of S. indica inhibited S. mutans and in case of biofilm assay, statistical analysis shows 55.22% of biofilm inhibition at a subinhibitory dose of 0.625mg/mL. Altogether, our investigations demonstrate that S. indica exhibits both antimicrobial and antibiofilm properties against S. mutans. Conclusion: Findings from the current study demonstrated that methanol extract derived from S. indica exhibit a antibacterial properties and significant reduction in biofilm formation were demonstrated through in vitro studies.