ISSN : 2663-2187

In-Vitro Antioxidant Activity of Bauhina variegata

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Aditya, Sonali, Pawan Jalwal, Shailja, Pankaj, Renu Malik, Rimmy, Arun Kumar
» doi: 10.33472/AFJBS.6.Si2.2024.2715-2731

Abstract

Bauhinia variegate, commonly known as Kachnar, belongs to family Leguminosae. The leaves of this plant used in the treatment of diabetes, ulcers, inflammation etc.Diabetes mellitus (DM) is denotation to a mixed group of a syndrome characterized by an elevated level of blood glucose due to imbalanced oxidation and utilization of glucose, which is related to the malfunction of insulin-producing pancreatic β-cells.The aim of present research is to study in-vitroantioxidant activity of Bauhinia variegata.The petroleum ether and methanol extracts prepared from the powdered leaves of Bauhinia variegata were subjected to qualitative phytochemical screening in order to check the presence of particular class of components present in them. The antioxidant activity of methanol extract of Bauhinia variegata against superoxide dismutase was found to be 58.64 and 95.8±0.4.The EC50 of methanol extract of Bauhinia variegata was 232.375 µg/ml respectively, and for ascorbic acid it was found to be 0.708 µg/ml. The IC50 of ascorbic acid was found to be 3.178 µg/ml whereas IC50 of methanol extract of Bauhinia variegata was calculated as 5.2 µg/ml. The radical scavenging ability of the compound is measured by stable radical DPPH (2, 2-diphenol-2-picryl hydrazyl). The DPPH scavenging activity of methanol extract at 5µg/ml was found to be 65.69 ± 0.10. The present study clearly indicated that B. variegata extract have appreciable in-vitro antioxidant effect

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