INTRODUCTION: Chronic periodontitis progressively disrupts periodontal tissues, eventually leading to tooth loss. In vertical bone loss defects, therapy includes surgical procedures, such as open flap debridement associated with scaffolds, grafts or membranes. The aim of the study is to fabricate a novel alginate, calcium iron silicate and ovine extracellular matrix based scaffold for periodontal regeneration MATERIALS AND METHODS: To 2% alginate solution 1mg/ml of CaFeSiO (calcium iron silicate) and ECM (extracellular matrix) was added to the solution. The solution is stirred to form homogeneous solution. The cross linked hydrogel was immersed in 0.15M CaCl2 solution. The Ca+ 2 ion diffuses into the gel and crosslink with the alginate. The cross-linked hydrogel is washed twice and stored at 4 ⁰C for further use. Scaffold characterisation was done by means of xray diffraction, raman spectrum and scanning electron microscope. Then hemocompatibility assay, swelling analysis, MTT compatibility test and differentiation test was done