This study aimed to explore the health benefits of RGLE, a herbal extract made from the leaves of Ruta graveolens, processed using methanol extraction. We conducted several in vitro tests to determine RGLE's potential in promoting health, particularly focusing on its ability to fight oxidative stress and support liver health. One of the well-known methods we used was the DPPH radical scavenging assay. The results were quite promising, showing that RGLE has a strong capability to neutralize harmful free radicals, which are known contributors to oxidative stress. Furthermore, we examined how RGLE could protect liver cells using a model where HepG2 cells were damaged with carbon tetrachloride, a common chemical used to study liver injury in the lab. The findings were encouraging—RGLE significantly protected these liver cells from damage caused by the chemical or toxicant, CCL4. Overall, our study indicated the functionality of RGLE as powerful antioxidant and a protector of liver cells. This suggests that RGLE could be a valuable natural option for managing oxidative stress and improving liver health. Further research, especially in clinical settings, could provide deeper insights into how RGLE can be used effectively for health enhancement and disease prevention.