A more advanced method for inducing microrhizomes in vitro in Alpinia galanga has been established. As the initial explants, newly sprouted shoot buds from rhizomes were used and replicated by in vitro techniques. In vitro produced shoots developed in MS medium fortified with cytokinin (BA) and various auxins (NAA, IAA and IBA). A maximum of shoots per explant were obtained inMS medium containing 0.5 mg/l BA and 1 mg/l IBA. Medium containing 1.0 mg/l BA in combination with 1.0 mg/l IAA showed the best response for induction of microrhizomes. The study further found that intermediate light (8 hr light and 16 hr darkness) greatly boosted the induction of microrhizomes, with 4% sucrose content being the optimal amount.Acclimatization of this microrhizome showed 55% of survivability rate in field condition.